Journal: Frontiers in Immunology
Article Title: Pan-cancer multi-omics characterization of calcyphosine and its revealed links to the immune microenvironment and regulatory networks in endometrial carcinoma
doi: 10.3389/fimmu.2025.1688606
Figure Lengend Snippet: CAPS expression in EC and its impact on cell proliferation. (A) Western blot analysis of CAPS protein levels in EC tissues and paired adjacent non-tumor tissues. (B) Quantification of CAPS protein expression based on Western blot results. (C) Representative immunohistochemical (IHC) staining images of CAPS in EC and adjacent tissues at 20× and 40× magnification. (D) Statistical analysis of IHC scores (n = 12 per group). (E, F) CCK-8 assays evaluating cell viability in KLE and Ishikawa cells following CAPS knockdown. (G, H) EdU staining assays assessing proliferative capacity after CAPS knockdown in KLE and Ishikawa cells. (I, J) Quantification of EdU-positive cells. (K) CAPS knockdown efficiency validated by Western blot in Ishikawa and KLE cells. CAPS protein levels were markedly reduced after transfection with Si1-CAPS and Si3-CAPS compared with Si-NC. β-actin served as the loading control.(* P < 0.05, ** P < 0.01, **** P < 0.0001).
Article Snippet: The human EC cell lines KLE and Ishikawa were obtained from GeneChem Co., Ltd. (Shanghai, China).
Techniques: Expressing, Western Blot, Immunohistochemical staining, Immunohistochemistry, CCK-8 Assay, Knockdown, Staining, Transfection, Control